Composite
Part:BBa_K1439008
Designed by: Zhaoliang Chen Group: iGEM14_OUC-China (2014-10-05)
TAT-H4-B0015
H4 histone can efficiently mediate gene transfer, it rich in positively charged alkaline amino acids and has the ability to combine with DNA. TAT-PTD contains an 11-amino acid peptide, and these amino acids YGRKKRRQRRR are highly basic, which is crucial for penetrating the plasma membrane. We use linker to link H4 histone and TAT, thus it can seize DNA and take it into cells. Now, it becomes our transfer device.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 260
Illegal XhoI site found at 268 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 177
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 241
[edit]
Categories
Parameters
None |